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Updated: Nov 16, 2025
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Altered circular RNA expression profiles in an ovalbumin-induced murine model of allergic rhinitis
Jie Chen1, Xiyan Xiao2, Shan He1
1Department of Otorhinolaryngology - Head and Neck Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.
Background:
Emerging evidence shows that circular RNAs (circRNAs) participate in the pathogenesis of multiple immune diseases. However, few studies have focused on the mechanisms of circRNAs involved in allergic rhinitis (AR).
Methods:
This study performed an RNA sequence (RNA-seq) profiling to identify the expression of circRNAs in nasal mucosa from ovalbumin-induced AR murine models and normal controls. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was then conducted to validate the differential expression of circRNAs. Bioinformatics analysis was applied to demonstrate the biological functions of the dysregulated circRNAs.
Results:
A total of 86 distinct circRNA candidates were sequenced, of which 51 were upregulated and 35 were downregulated. The T cell receptor, B cell receptor, and calcium signaling pathways may be involved in the pathology of AR. Furthermore, a circRNA-miRNA interaction network was constructed via miRNA response elements analysis. Some circRNAs were correlated with miRNAs that are involved in T cell polarization and activation, thereby highlighting their potential role in the pathogenesis of AR.
Conclusions:
This study demonstrates a number of aberrantly expressed circRNAs related to AR, and offers a novel perspective into AR pathogenesis and future therapeutic strategies.
Insights
Circular RNAs (circRNAs) show altered expression in allergic rhinitis (AR). These findings reveal new insights into AR pathogenesis and potential therapeutic targets.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are implicated in various immune diseases.
- The role of circRNAs in allergic rhinitis (AR) pathogenesis remains largely unexplored.
Purpose of the Study:
- To investigate the expression profiles and potential functions of circRNAs in AR.
- To identify novel circRNAs involved in the molecular mechanisms of AR.
Main Methods:
- RNA sequencing (RNA-seq) was used to profile circRNAs in nasal mucosa of AR mouse models and controls.
- Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) validated differential circRNA expression.
- Bioinformatics analysis explored the functions and interactions of dysregulated circRNAs.
Main Results:
- 86 distinct circRNAs were identified, with 51 upregulated and 35 downregulated in AR.
- Potential involvement of T cell receptor, B cell receptor, and calcium signaling pathways in AR pathology.
- A circRNA-miRNA network revealed correlations between circRNAs and miRNAs involved in T cell polarization and activation.
Conclusions:
- Aberrantly expressed circRNAs are associated with allergic rhinitis.
- This study provides a novel perspective on AR pathogenesis and potential therapeutic strategies targeting circRNAs.
