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Expression of endoplasmic reticulum stress mRNAs in otitis media

Su Young Jung1, Ki Jin Kwon2, Hye Kyu Min2

  • 1Department of Otorhinolaryngology - Head and Neck Surgery, Myongji Hospital, Hanyang University College of Medicine, Goyang, Korea.

Acta Oto-Laryngologica
|March 1, 2021
PubMed
Abstract

Insights

Endoplasmic reticulum (ER) stress plays a role in otitis media. Different ER stress mRNA levels were observed in otitis media with effusion (OME), chronic otitis media (COM), and cholesteatoma (CholeOM) based on bacterial culture results.

Area of Science:

  • Otolaryngology
  • Cellular Biology
  • Molecular Medicine

Background:

  • Endoplasmic reticulum (ER) stress is a cellular response to impaired ER function.
  • Investigating ER stress provides insight into cellular defense mechanisms.

Purpose of the Study:

  • To evaluate the expression of key endoplasmic reticulum (ER) stress-related mRNAs in patients with otitis media with effusion (OME), chronic otitis media (COM), and COM with cholesteatoma (CholeOM).

Main Methods:

  • Surgical specimens from OME, COM, and CholeOM patients were analyzed.
  • Real-time polymerase chain reaction (PCR) was used to quantify ER stress mRNA levels.
  • mRNA levels were compared across disease types and correlated with clinical data and bacterial culture findings.

Main Results:

  • CHOP mRNA levels were elevated, while sXBP1 and ATF6 mRNA levels were reduced in OME compared to COM and CholeOM.
  • In bacterial pus culture-negative patients, ATF6 mRNA was higher in CholeOM.
  • In bacterial pus culture-positive patients, CHOP mRNA was higher in OME.

Conclusions:

  • Endoplasmic reticulum (ER) stress is implicated in the pathophysiology of otitis media (OM).
  • Differential expression of ER stress mRNAs correlates with specific otitis media subtypes and bacterial presence.
Keywords:
Chronic otitis media, CholesteatomaEndoplasmic reticulum stress

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