Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Remodelling of miRNA Regulatory Landscape During West Nile Virus (WNV) Infection.

Epigenomes·2026

Same author

Safety, immunogenicity, and dose ranging of a bivalent respiratory syncytial virus and human metapneumovirus glycoprotein F-molecular clamp vaccine candidate in older adults: 1-month interim analysis of an ongoing randomised, observer-blind, placebo- and active-controlled, phase 1 trial.

Vaccine·2026

Same author

Unique molecular profiling of monocyte responses to a high dose of DENV-NS1 reflected the effect of NS1 on hemostasis.

Medical microbiology and immunology·2026

Same author

Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress.

Nature communications·2026

Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026

Same author

IgG3 Hinge Architecture Expands Dengue Virus Breadth of A Quaternary Epitope Dependent Zika Virus Cross-Neutralizing Antibody.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Nov 16, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

An Optimized High-Throughput Immuno-Plaque Assay for SARS-CoV-2.

Alberto A Amarilla¹, Naphak Modhiran^1,2, Yin Xiang Setoh¹

¹School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.

Frontiers in Microbiology

|March 1, 2021

Summary

A new immuno-plaque assay (iPA) offers rapid, sensitive detection and quantification of SARS-CoV-2. This method accelerates crucial research for coronavirus disease 2019 by enabling ultra-high-throughput viral titration and neutralization assays.

Keywords:

SARS-CoV-2 coronaviruses immuno-plaque assay (iPA)viral quantification

More Related Videos

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Related Experiment Videos

Last Updated: Nov 16, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Area of Science:

Virology
Immunology
Assay Development

Background:

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19).
Rapid, sensitive, and reliable viral detection is critical due to global spread.
Existing methodologies may require optimization for speed and throughput.

Purpose of the Study:

To develop and optimize an alternative assay for SARS-CoV-2 detection and quantification.
To demonstrate the flexibility and high-throughput capabilities of the novel assay.
To accelerate SARS-CoV-2 research during the pandemic.

Main Methods:

Development of an immuno-plaque assay (iPA) combining plaque assay and immunofluorescence.
Optimization of SARS-CoV-2 infection conditions for the iPA.
Demonstration of iPA flexibility using multiple antibodies and dual-probing.

Main Results:

The iPA is highly flexible, allowing detection with various antibodies and dual-epitope probing.
The assay is suitable for ultra-high-throughput viral titration and neutralization assays.
iPA is compatible with a 384-well format and can be completed within 24 hours.

Conclusions:

The immuno-plaque assay (iPA) provides a rapid, sensitive, and flexible method for SARS-CoV-2 detection.
iPA's high-throughput capability significantly accelerates research on SARS-CoV-2.
This assay is a valuable tool for advancing COVID-19 research and diagnostics.