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RAMP2-AS1 Regulates Endothelial Homeostasis and Aging
Chih-Hung Lai1,2,3, Aleysha T Chen1, Andrew B Burns1
1Department of Diabetes Complications and Metabolism, City of Hope, Duarte, CA, United States.
Frontiers in Cell and Developmental Biology
|March 1, 2021
Summary
Vascular endothelium homeostasis is vital for heart health. A novel long non-coding RNA, RAMP2-AS1, is identified as crucial for maintaining endothelial cell function and preventing aging.
Area of Science:
- Vascular biology
- Molecular biology
- Genomics
Background:
- Endothelial cell (EC) aging and dysfunction impair vascular health.
- Maintaining vascular endothelium homeostasis is critical for cardiovascular well-being.
Purpose of the Study:
- To identify key regulators of endothelial cell homeostasis and aging using transcriptomic data.
- To investigate the role of long non-coding RNAs (lncRNAs) in EC function and aging.
Main Methods:
- Transcriptome profiling of ECs under various conditions, including physiological and pathophysiological flow.
- Principal Component Analysis (PCA) to identify key genes associated with EC transcriptional states.
- Bioinformatics analysis to identify novel regulatory lncRNAs, including RAMP2-AS1.
Main Results:
- Identified RAMP2-AS1, a lncRNA antisense to RAMP2, as a novel regulator of EC homeostasis.
- Knockdown of RAMP2-AS1 decreased RAMP2 expression, promoted EC aging phenotypes like impaired angiogenesis and increased senescence.
- Developed an integrative approach to quantify EC aging based on transcriptome changes.
Conclusions:
- RAMP2-AS1 is essential for maintaining EC homeostasis and function, and its dysregulation contributes to EC aging.
- Transcriptome analysis is a powerful tool for discovering novel regulators of EC function and aging.
- Identified RAMP2-AS1 as a potential therapeutic target for vascular aging and dysfunction.
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