[Recent advances of continuous in vivo evolution].

Area of Science:

• Microbiology
• Biotechnology
• Synthetic Biology

Background:

• Laboratory evolution is crucial for enhancing microorganism performance but traditional methods are labor-intensive and lack continuous mutation.
• Existing strategies for strain or protein evolution require multiple rounds, limiting efficiency.

Purpose of the Study:

• To review recent advancements in continuous in vivo evolution technologies.
• To summarize principles, applications, advantages, and limitations of these technologies.
• To provide a future outlook on continuous in vivo evolution.

Main Methods:

• Review of recent literature on continuous in vivo evolution.
• Analysis of technologies enabling genome-scale mutation and specific protein evolution.
• Discussion of integrated mutation and screening processes.

Main Results:

• Continuous in vivo evolution integrates mutation and screening for efficient phenotype evolution.
• Technologies now allow for genome-scale mutation and targeted protein evolution.
• These methods significantly reduce human intervention and improve laboratory evolution efficiency.

Conclusions:

• Continuous in vivo evolution represents a significant leap in laboratory evolution efficiency.
• The integration of mutation and screening within a single process is key.
• Future developments are expected to further refine these powerful biotechnological tools.