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Updated: Nov 15, 2025

A Murine Tail Lymphedema Model
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A Murine Tail Lymphedema Model

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A Murine Tail Lymphedema Model.

Aladdin H Hassanein1, Mithun Sinha2, Colby R Neumann2

  • 1Department of Surgery, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine; ahassane@iu.edu.

Journal of Visualized Experiments : Jove
|March 1, 2021
PubMed
Summary
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Researchers improved a mouse model for lymphedema, a lymphatic dysfunction causing limb swelling. This enhanced model provides sustained swelling and reliable perfusion, improving its clinical relevance for studying the incurable disease.

Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Lymphatic Research

Background:

  • Lymphedema, characterized by limb swelling due to lymphatic dysfunction, currently lacks a cure.
  • Traditional mouse tail models for lymphedema exhibit limitations, including vascular compromise and premature swelling resolution, hindering clinical translatability.
  • Developing a reliable and clinically relevant animal model is crucial for advancing lymphedema research.

Purpose of the Study:

  • To enhance the traditional murine tail lymphedema model for improved clinical translatability.
  • To establish a chronic model inducing sustained lymphedema and reliable tail perfusion.
  • To incorporate advanced techniques for precise surgical intervention and functional assessment.

Main Methods:

  • Precise full-thickness skin excision and lymphatic clipping utilizing a surgical microscope.

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Last Updated: Nov 15, 2025

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  • High-resolution laser speckle imaging to confirm post-operative arterial and venous perfusion.
  • Indocyanine green near-infrared laser lymphangiography for functional assessment of lymphatic function.
  • Application of tissue nanotransfection technology (TNT) for non-viral, transcutaneous genetic cargo delivery.

    • Main Results:

    • The enhanced model successfully induces sustained lymphedema in mice over a 15-week period.
    • Reliable arterial and venous perfusion to the tail was confirmed post-surgery.
    • The model demonstrates improved precision and functional assessment capabilities compared to traditional methods.
    • Successful delivery of genetic cargo via TNT was achieved.

    Conclusions:

    • The enhanced chronic murine tail lymphedema model offers a more clinically translatable platform for studying lymphedema.
    • This improved model facilitates research into therapeutic strategies for lymphedema by providing sustained disease manifestation and reliable assessment tools.
    • The integration of TNT presents a novel approach for targeted genetic therapies in lymphedema research.