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Programming folding cooperativity of the dimeric i-motif with DNA frameworks for sensing small pH variations
Shiliang He1, Mengmeng Liu, Fangfei Yin
1Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Chemical Communications (Cambridge, England)
|March 1, 2021
Summary
Researchers enhanced DNA i-motif folding cooperativity using a DNA framework. This innovation led to the development of highly sensitive intracellular pH sensors.
Area of Science:
- Molecular Biology
- Biotechnology
- Nanotechnology
Background:
- The sensitivity of molecular sensors is linked to the folding cooperativity of their responsive elements.
- DNA i-motifs are pH-responsive structures with potential applications in biosensing.
Purpose of the Study:
- To enhance the folding cooperativity of a hydrogen ion (H+) responsive dimeric DNA i-motif.
- To develop robust intracellular pH sensors with improved response sensitivity.
Main Methods:
- Utilized a DNA framework to preorganize a dimeric DNA i-motif structure.
- Investigated the impact of preorganization on folding cooperativity.
- Fabricated and tested intracellular pH sensors based on the modified i-motif.
Main Results:
- Demonstrated successful enhancement of folding cooperativity through DNA framework preorganization.
- Achieved fabrication of robust intracellular pH sensors.
- The developed sensors exhibited high response sensitivity to intracellular pH changes.
Conclusions:
- Preorganization via a DNA framework is an effective strategy to boost the folding cooperativity of DNA i-motifs.
- This approach enables the creation of advanced, highly sensitive biosensors for intracellular pH monitoring.
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