Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individualised treatment effects of corticosteroids in IgA nephropathy.

EBioMedicine·2026
Same author

Next-generation kidney tissue analysis - spatial omics and digital pathology.

Nature reviews. Nephrology·2026
Same author

Artificial Intelligence in Kidney Transplant Pathology: Current Evidence, Limitations, and Relevance to the Banff Framework.

Kidney international reports·2026
Same author

Evaluating and Mitigating Carbon Dioxide Equivalent Emissions in Stroke Management: A Modeling Study.

Journal of the American Heart Association·2026
Same author

The role of platelet-derived growth factor in uremic vascular calcification.

Kidney international·2026
Same author

Strategic timing of contralateral nephrectomy after ischemic acute kidney injury prevents chronic kidney disease by enhancing progenitor proliferation, attenuating polyploidization and reshaping the immune response.

Kidney international·2026

Related Experiment Video

Updated: Nov 15, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.0K

[Methods of SARS-CoV-2 detection in tissue].

Saskia von Stillfried1, Peter Boor2,3

  • 1Institut für Pathologie, Universitätsklinik der RWTH Aachen, Pauwelsstr. 30, 52074, Aachen, Deutschland.

Der Pathologe
|March 1, 2021
PubMed
Summary

Detecting SARS-CoV-2 in tissues is crucial for understanding COVID-19. RNA detection methods, like RT-PCR, show higher sensitivity and specificity than protein detection, making them the current method of choice.

Keywords:
COVID-19Electron microscopyFluorescence in situ hybridizationPreanalytical phaseReverse transcriptase polymerase chain reaction

More Related Videos

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.3K

Related Experiment Videos

Last Updated: Nov 15, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.0K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

5.0K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.3K

Area of Science:

  • Virology
  • Pathology
  • Molecular Biology

Background:

  • SARS-CoV-2 detection in patient tissues is vital for understanding COVID-19 pathophysiology.
  • Accurate interpretation of histopathological findings in autopsies, biopsies, and surgical specimens is essential.
  • Assessing occupational infectious hazards requires reliable tissue analysis.

Purpose of the Study:

  • To review and evaluate SARS-CoV-2 detection methods in human tissues.
  • To identify sources of error, assess specificity, and determine sensitivity of various detection techniques.
  • To provide recommendations for optimal SARS-CoV-2 tissue analysis.

Main Methods:

  • A comprehensive literature review of 136 studies from PubMed's LitCovid database.
  • Evaluation of SARS-CoV-2 detection methods including RNA, protein/antigen, and electron microscopy.
  • Analysis of preanalytical factors, particularly tissue quality and post-mortem intervals.

Main Results:

  • No specific histomorphological features for COVID-19 are currently established.
  • Tissue quality and preanalytical handling are significant sources of error, especially in autopsy samples.
  • RNA detection methods (RT-PCR) demonstrate superior sensitivity and specificity compared to protein/antigen detection and electron microscopy.

Conclusions:

  • Various methods exist for SARS-CoV-2 tissue detection, with RNA detection via RT-PCR being the preferred approach.
  • Extensive validation and harmonization of detection methods are critically needed.
  • Standardized protocols are essential for reliable and reproducible SARS-CoV-2 tissue analysis.