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BC-store: A program for MGISEQ barcode sets analysis.

Irina Bulusheva1, Vera Belova1, Boris Nikashin1

  • 1Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, Russia.

Plos One
|March 1, 2021
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Summary

BC-store is a new open-source program designed for analyzing and selecting compatible barcode sets for MGI sequencing platforms. This tool enhances sequence lab efficiency by simplifying barcode compatibility analysis and management.

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Area of Science:

  • Bioinformatics
  • Genomic Sequencing

Background:

  • High-throughput sequencing platforms, such as those from MGI Tech, rely on accurate barcode identification.
  • Efficient selection and analysis of barcode sets are crucial for successful sequencing experiments.

Purpose of the Study:

  • To introduce BC-store, a novel software tool for analyzing and selecting barcode sets.
  • To facilitate the use of MGI Tech sequencing platforms through improved barcode management.

Main Methods:

  • Development of BC-store as an open-source program in Python 3.
  • Implementation of features for analyzing barcode compatibility within a flow cell lane.
  • Inclusion of functionality for adding barcodes to existing sets with custom sharing.

Main Results:

  • BC-store provides a method for analyzing barcode compatibility for equal and arbitrary fractions.
  • The program supports adding barcodes to existing sets with customizable sharing options.
  • Demonstration of BC-store's effectiveness in routine sequence laboratory tasks.

Conclusions:

  • BC-store is an effective tool for optimizing barcode selection and analysis for MGI sequencing.
  • The software improves the efficiency and reliability of sequencing workflows in research labs.