The toxicological mechanism of two typical imidazole ionic liquids in textile industry on Isatis tinctoria

Ionic liquids (ILs¹) which are called "green solvents", are used widely in the textile industry as adjuvants due to their many advantages. However, their persistent residues may cause ecotoxicity. The aim of the study is to explore the toxicity of different anions on imidazole ILs and their toxicological mechanism. For the experiments 1-butyl-3-methylimidazole tetrafloroborate ([C₄mim]BF₄) and 1- butyl -3-methylimidazolium chloride ([C₄mim]Cl) were selected to study their toxic effects on Isatis tinctoria. ILs may affect the germination rate. Fresh weight, dry weight and Hill reaction activity decreased continuously with increasing of IL concentrations, showing an effect-dose relationship. Transmission electron microscopy (TEM) revealed that cell walls were fuzzy, starch granules had accumulated and the chloroplast structure was damaged. These changes will affected the function and electron transport efficiency of photosystemⅡ. Superoxide anion accumulation stimulated the activity of antioxidant enzymes (SOD, POD, CAT) and caused lipid peroxidation as well as an increased malondialdehyde content. ILs also reduced indirubin and total flavonoids contents, which reduced the pharmacological efficacy of Isatis tinctoria. This is demonstrated by three-dimensional fluorescence chromatogram. [C₄mim]Cl was more toxic than [C₄mim]BF₄. ILs caused toxic effects to Isatis tinctoria. The ecological toxicity of ILs should be considered when using them as additives in the textile industry.