Ex Vivo Complement Activation on Endothelial Cells: Research and Translational Value
Marie-Sophie Meuleman1, Veronique Fremeaux-Bacchi1, Lubka T Roumenina1
1Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université de Paris, F-75006 Paris, France.
Trends in Molecular Medicine
|March 2, 2021
Summary
Researchers developed a new assay to measure complement activation on endothelial cells. This tool addresses the need for better methods to study complement
Area of Science:
- Immunology
- Vascular Biology
- Drug Discovery
Background:
- The complement system plays an increasing role in various human diseases.
- Current methods to assess complement activation and evaluate complement inhibitors are insufficient for clinical application.
Purpose of the Study:
- To present a novel functional ex vivo assay for monitoring complement activation.
- To address the need for better tools in complement research and therapeutic development.
Main Methods:
- Development and validation of an ex vivo assay.
- Assay focuses on monitoring complement activation specifically on endothelial cells.
Main Results:
- The described assay provides a functional method to assess complement activation.
- This assay can be utilized to study complement's role in disease and evaluate potential inhibitors.
Conclusions:
- A new ex vivo assay effectively monitors complement activation on endothelial cells.
- This assay meets the demand for improved tools in complementology and the development of complement inhibitors for clinical use.
