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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

6.9K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Related Experiment Video

Updated: Nov 15, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

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Preparing Frozen Tissue Sections for Staining.

Scott J Rodig

    Cold Spring Harbor Protocols
    |March 2, 2021
    PubMed
    Summary
    This summary is machine-generated.

    Frozen sections offer gentle mammalian tissue preparation for cell staining, preserving structure and antigens. However, they require frozen storage, specialized cryostat equipment, and are less common than formalin-fixed, paraffin-embedded tissues.

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    Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

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    Area of Science:

    • Histology
    • Immunohistochemistry
    • Mammalian tissue analysis

    Background:

    • Cell-staining studies frequently utilize frozen tissue sections for optimal sample preparation.
    • Frozen sections provide superior preservation of cellular structure and antigenicity.
    • Key limitations include the necessity for frozen storage, specialized cryostat equipment, and limited availability of clinical specimens in this format.

    Purpose of the Study:

    • To highlight the advantages of frozen sections in cell-staining studies.
    • To discuss the disadvantages and limitations associated with frozen section preparation and usage.
    • To contrast frozen sections with formalin-fixed, paraffin-embedded (FFPE) tissues, which form the basis of classical histopathology.

    Main Methods:

    • Review of established protocols for frozen section preparation.
    • Comparison of antigen and structural preservation between frozen and FFPE tissues.
    • Analysis of the practical requirements and limitations for using frozen sections in research and clinical settings.

    Main Results:

    • Frozen sections offer excellent preservation of cellular morphology and antigenicity, making them ideal for sensitive staining techniques.
    • The requirement for continuous frozen storage and specialized cryostat equipment presents logistical challenges.
    • Many clinical samples are not readily available as frozen sections, and classical histopathology relies on FFPE material.

    Conclusions:

    • Frozen sections are a valuable method for preserving tissue integrity and antigenicity in cell-staining studies.
    • The practical constraints of frozen storage and equipment, alongside the prevalence of FFPE samples, necessitate careful consideration of tissue preparation methods.
    • Understanding the trade-offs between frozen and FFPE tissues is crucial for selecting the most appropriate technique for specific research and diagnostic applications.