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Related Experiment Video

Updated: Nov 15, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Progenitor cell diversity in the developing mouse neocortex.

Xiangbin Ruan¹, Bowei Kang^1,2, Cai Qi¹

¹Department of Human Genetics, The University of Chicago, Chicago, IL 60637.

Proceedings of the National Academy of Sciences of the United States of America

|March 2, 2021

Summary

This summary is machine-generated.

Temporal gene expression in neural progenitors correlates with neuron type specification in the developing mouse neocortex. This study reveals that Eomes-positive cells show transcriptionally evident neuronal identity genes.

Keywords:

cell identity developmental timing neural progenitor neuronal diversity scRNA-Seq

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Last Updated: Nov 15, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

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Published on: June 4, 2014

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Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

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Published on: March 8, 2024

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Mammalian neocortex projection neuron types arise sequentially from a common progenitor pool.
The relationship between developmental timing and neuron type specification is not fully understood.

Purpose of the Study:

To investigate the correlation between temporal gene expression in neural progenitors and neuron type specification.
To delineate neural progenitor cell diversity in the developing mouse neocortex.

Main Methods:

Single-cell RNA sequencing (scRNA-Seq) of the developing mouse neocortex.
Analysis of gene expression patterns in neuroepithelial cells, radial glial cells (RGCs), and intermediate progenitor cells.

Main Results:

Identified neuroepithelial cell-enriched genes (e.g., Hmga2, Ccnd1).
Observed dynamic gene expression in RGCs over time (e.g., Hes5 in early RGCs, Pou3f2 in late RGCs).
Found that the intermediate progenitor cell marker Eomes coexpresses temporally with neuronal identity genes, particularly in postmitotic cells.

Conclusions:

Neural progenitor cell diversity exists in the developing mouse neocortex.
Neuronal identity genes are transcriptionally evident in Eomes-positive cells, supporting a role in temporal specification.