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Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis
Oh Sung Kwon1, Rahul Mishra1,2, Adham Safieddine3,4
1Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France.
Nature Communications
|March 2, 2021
Summary
Exon junction complexes (EJCs) accumulate at cell basal bodies in quiescent stem cells. This EJC accumulation is crucial for NIN mRNA localization, impacting cell division and brain development.
Area of Science:
- Cell Biology
- Molecular Biology
- Neuroscience
Background:
- Exon junction complexes (EJCs) are vital for mRNA processing and function.
- EJC dosage imbalance is implicated in neurodevelopmental disorders.
- Centrioles serve as basal bodies for ciliogenesis in quiescent cells.
Purpose of the Study:
- Investigate the role of EJCs in quiescent cell biology.
- Identify mRNA molecules localized to centrosomes and basal bodies.
- Determine the impact of EJC dysfunction on cell division and development.
Main Methods:
- High-throughput smFISH screening to identify localized transcripts.
- Analysis of EJC localization in mouse neural stem cells (mNSCs) and RPE1 cells.
- Assessment of ciliogenesis and pericentriolar material organization.
Main Results:
- EJCs accumulate at basal bodies in quiescent mNSCs and RPE1 cells, decreasing upon differentiation.
- NIN and BICD2 transcripts are identified at centrosomes in quiescent cells.
- NIN mRNA localization is EJC-dependent, and EJC downregulation impairs ciliogenesis and pericentriolar material organization.
Conclusions:
- EJC-dependent mRNA trafficking to centrosomes and basal bodies is essential for cell function.
- Proper EJC function supports mNSC division and brain development.
- Findings reveal a novel role for EJCs in regulating mRNA localization for cellular processes.
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