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Biglycan expression and its function in human ligamentum flavum
Hamidullah Salimi1, Akinobu Suzuki2, Hasibullah Habibi1
1Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahi Machi, Abeno-Ku, Osaka, 545-8585, Japan.
Scientific Reports
|March 2, 2021
Summary
Biglycan (BGN), a proteoglycan, is overexpressed in ligamentum flavum (LF) hypertrophy causing lumbar spinal stenosis (LSS). Increased BGN promotes cell proliferation, differentiation, and migration, contributing to LF pathology.
Area of Science:
- Biochemistry
- Cell Biology
- Orthopedics
Background:
- Ligamentum flavum (LF) hypertrophy is a primary cause of lumbar spinal stenosis (LSS).
- Proteoglycan family members are implicated in LF hypertrophy.
- Biglycan (BGN) is upregulated in a rabbit model of LF hypertrophy due to mechanical stress.
Purpose of the Study:
- To investigate the role and expression of Biglycan (BGN) in human LF hypertrophy.
- To elucidate the functional involvement of BGN in the pathomechanism of human LF hypertrophy.
Main Methods:
- Immunohistochemistry was used to confirm BGN expression in human LF tissues.
- Primary cell cultures of LF cells (hypertrophied and non-hypertrophied) were used to assess BGN function.
Main Results:
- BGN expression was significantly increased in the extracellular matrix of hypertrophied LF from LSS patients.
- BGN treatment promoted proliferation, altered cell morphology, and enhanced myofibroblastic differentiation and migration in LF cells.
- These effects were observed in both hypertrophied and non-hypertrophied LF cells.
Conclusions:
- This study demonstrates hyper-expression of BGN in hypertrophied human LF.
- Increased BGN contributes to LF hypertrophy by promoting cell proliferation, myofibroblastic differentiation, and cell migration.
- BGN is a key player in the pathophysiology of LF hypertrophy.
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