Ethanol-induced modifications to membrane lipid structure: effect on phospholipase A2-membrane interactions.

¹Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.

|May 1, 1988

Summary

Chronic ethanol intake causes "membrane tolerance," a resistance to lipid changes. This study shows that ethanol-treated rats develop altered liver microsome lipid structures, making them resistant to phospholipase A2 action.

Area of Science:

Biochemistry
Cell Biology
Pharmacology

Background:

Chronic ethanol intoxication induces
membrane tolerance,
a resistance to ethanol's lipid-disordering effects.
Existing spectroscopic methods indirectly detect this tolerance, raising questions about underlying lipid structural changes.

Purpose of the Study:

To investigate whether chronic ethanol ingestion alters membrane lipid structure.
To explore the mechanism of ethanol-induced membrane tolerance.

Main Methods:

Assessing resistance to exogenous phospholipase A2 hydrolysis in liver microsomes from chronically ethanol-treated and control rats.
Examining reconstituted lipid vesicles prepared from extracted phospholipids.
Utilizing a specific phospholipid substrate (1-palmitoyl-2-N-(4-nitrobenzo-2-oxa-1,3-diazole)amino caproylphosphatidylcholine).
Investigating the effect of oleic acid on phospholipase A2 resistance.

Main Results:

Liver microsomes from ethanol-treated rats showed relative resistance to phospholipase A2.
This resistance was maintained in reconstituted lipid vesicles.
Increasing oleic acid levels progressively reduced the ethanol-induced resistance to hydrolysis.

Conclusions:

Chronic ethanol treatment modifies membrane lipid structure, likely at the bilayer surface.
Exogenous phospholipase A2 serves as a novel tool for probing ethanol-induced structural modifications.
Membrane tolerance involves alterations in lipid structure, not just indirect detection.

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Ethanol-induced modifications to membrane lipid structure: effect on phospholipase A2-membrane interactions.

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

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