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Updated: Nov 15, 2025
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Gain of circBRAF Represses Glioma Progression by Regulating miR-1290/FBXW7 Axis
Jinchao Zhang1, Zhi Chen2, Xinjun Liu2
1Department of Neurosurgery, Yingtan People's Hospital, No. 116, Shenglixi Road, Yingtan, 335000, Jiangxi, China. cksfwn@163.com.
Abstract:
Dysregulated circular RNAs (circRNAs) have been confirmed to partake in the modulation of the glioma progression. Here, we intended to explore the role of circBRAF in glioma and the possible action mechanism. The expression levels of circBRAF, microRNA (miR)-1290 and F-box and WD repeat domain containing 7 (FBXW7) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or western blot. Cell viability was assessed by 3-(4, 5)-dimethylthiazole-2-y1)-2, 5-biphenyl tetrazolium bromide (MTT) assay. Cell cycle distribution was determined by flow cytometry. Cell migration and invasion were evaluated through Trans-well assay. Related protein levels were detected by western blot. Targeted relation among circBRAF, miR-1290 and FBXW7 was validated by dual-luciferase reporter, RNA immunoprecipitation (RIP) and pull-down assays. Xenograft model was constructed to explore the function of circBRAF in vivo. Expression of circBRAF and FBXW7 was decreased in glioma tissues and cells. Upregulation of circBRAF inhibited glioma cell proliferation and metastasis in vitro. MiR-1290 was upregulated in glioma, which was sponged by circBRAF. Besides, circBRAF elevated FBXW7 expression by targeting miR-1290. Introduction of miR-1290 or FBXW7 knockdown could counteract the inhibitory effects of circBRAF upregulation on the malignant phenotypes of glioma cells. Overexpression of circBRAF repressed the tumor growth in vivo. Upregulation of circBRAF suppressed glioma evolvement in vitro and in vivo by regulating miR-1290/FBXW7 axis, broadening the cognition of glioma progression.
Insights
Circular RNA BRAF (circBRAF) suppresses glioma progression by inhibiting cell proliferation and metastasis. It achieves this by sponging microRNA-1290, thereby increasing F-box and WD repeat domain containing 7 (FBXW7) expression, impacting tumor growth.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Dysregulated circular RNAs (circRNAs) are implicated in glioma progression.
- Understanding the specific roles of circRNAs, like circBRAF, is crucial for glioma research.
Purpose of the Study:
- To investigate the function of circBRAF in glioma.
- To elucidate the underlying molecular mechanism involving microRNA-1290 (miR-1290) and F-box and WD repeat domain containing 7 (FBXW7).
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot for expression analysis.
- In vitro assays (MTT, flow cytometry, Trans-well) for cell proliferation, cycle, migration, and invasion.
- Dual-luciferase reporter, RNA immunoprecipitation (RIP), and pull-down assays for mechanism validation.
- Xenograft models for in vivo studies.
Main Results:
- circBRAF and FBXW7 expression were decreased in glioma tissues and cells.
- circBRAF upregulation inhibited glioma cell proliferation and metastasis in vitro.
- circBRAF sponged miR-1290, leading to increased FBXW7 expression.
- Overexpression of circBRAF repressed tumor growth in vivo.
Conclusions:
- circBRAF suppresses glioma progression by regulating the miR-1290/FBXW7 axis.
- This finding expands the understanding of glioma pathogenesis and potential therapeutic targets.
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