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Updated: Nov 15, 2025
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
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circUBAP2 regulates osteosarcoma progression via the miR‑204‑3p/HMGA2 axis
Weiguo Ma1, Ning Xue1, Junhua Zhang1
1Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan 450008, P.R. China.
International Journal of Oncology
|March 2, 2021
Summary
Circular RNA UBAP2 (circUBAP2) is upregulated in osteosarcoma, promoting cancer cell growth and invasion. Inhibiting circUBAP2 via the miR-204-3p/HMGA2 pathway suppressed osteosarcoma progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNA UBAP2 (circUBAP2) is implicated in various cancers, but its role in osteosarcoma (OS) remains unclear.
- Understanding the molecular mechanisms of circUBAP2 in OS is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the expression, function, and molecular mechanism of circUBAP2 in osteosarcoma.
- To elucidate the relationship between circUBAP2, miR-204-3p, and HMGA2 in osteosarcoma progression.
Main Methods:
- Quantitative PCR to assess circUBAP2, miR-204-3p, and HMGA2 expression.
- Cell proliferation, migration, invasion, and apoptosis assays (CCK-8, Transwell, flow cytometry).
- Dual-luciferase reporter assays and RNA immunoprecipitation (RIP) to confirm molecular interactions.
Main Results:
- circUBAP2 was significantly upregulated in OS tissues and cell lines, correlating with poorer survival.
- circUBAP2 knockdown inhibited OS cell proliferation, migration, and invasion, while promoting apoptosis.
- circUBAP2 directly targets miR-204-3p, which in turn targets HMGA2, forming the circUBAP2/miR-204-3p/HMGA2 axis.
Conclusions:
- circUBAP2 promotes osteosarcoma progression by sponging miR-204-3p and upregulating HMGA2.
- The circUBAP2/miR-204-3p/HMGA2 axis represents a potential therapeutic target for osteosarcoma treatment.
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