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Related Experiment Video

Updated: Nov 15, 2025

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

Standard protocol for mass photometry experiments.

Di Wu¹, Grzegorz Piszczek²

¹Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.

European Biophysics Journal : EBJ

|March 2, 2021

Summary

This summary is machine-generated.

Mass photometry (MP) is a powerful technique for measuring molecular mass distributions of biomolecules in solution. This protocol offers guidance for new and experienced users to ensure consistent and accurate mass photometry experiments.

Keywords:

Label-free Mass distribution Mass photometry Protein characterization Single-molecule

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Area of Science:

Biophysics
Analytical Chemistry
Biochemistry

Background:

Mass photometry (MP) is an emerging experimental technique for determining the mass of individual molecules in solution.
Its applications are rapidly expanding due to its speed, sensitivity, and label-free detection capabilities.

Purpose of the Study:

To present a comprehensive protocol for determining protein molecular mass distributions using mass photometry.
To provide guidance for new users and a checklist for experienced laboratories to ensure consistent data collection and documentation.

Main Methods:

Detailed description of sample and materials preparation for MP experiments.
Guidelines for data collection and analysis specific to protein mass determination.
Discussion of the advantages, limitations, and potential artifacts associated with MP.

Main Results:

The protocol facilitates the accurate determination of molecular mass distributions for proteins and other biomolecules.
It addresses challenges faced by new users of single-molecule techniques.
The presented method aids in consistent data collection and documentation for routine MP analysis.

Conclusions:

This protocol serves as a valuable resource for implementing and optimizing mass photometry experiments.
It empowers researchers to reliably measure molecular masses and distributions in solution.
Consistent application of this protocol will enhance the reproducibility and comparability of MP data across laboratories.