Related Experiment Video
Updated: Nov 15, 2025
Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
Analysis of Copy Number Variation in Urine: c-Myc Evaluation Using a Real-Time PCR Approach
Valentina Casadio1, Filippo Martignano2,3, Roberta Gunelli4
1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
Abstract:
Urine cell-free DNA has been shown as an informative noninvasive source of biomarkers for a number of diseases, especially for urological cancers. Starting from the hypothesis that the gain of c-Myc gene is a frequent aberration in several cancer types, including prostate cancer, we analyzed c-Myc copy number variation in urine, studying a little case series of prostate cancer patients, to test its feasibility. Here we report a general protocol that may be considered to analyze gene copy number variation in the urine cell-free fraction.
Related Concept Videos
02:26Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
02:57Real Time RT-PCR
The real-time quantification of the number of amplified products is...