Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular vesicles released by uterine fibroid cells: modulation by omega-3 fatty acids and impact on recipient myometrial cells.

F&S science·2026
Same author

Effects of combined Mediterranean diet and physical activity intervention on the gut microbiome and disease progression in individuals with Parkinson's disease: study protocol for a multicenter, randomized controlled pilot study (PRIME study).

Frontiers in aging neuroscience·2026
Same author

Gut Microbiota Profile and the Impact of Probiotic Supplementation in Competitive Cyclists: A Scoping Review.

Nutrients·2026
Same author

Are glutathionylated aldehyde reductases the missing piece of the "catecholaldehyde hypothesis" in Parkinson's disease? A medical hypothesis concerning the detoxification of 4-hydroxynonenal (HNE) and 3,4-dihydroxyphenylacetaldehyde (DOPAL).

Redox biology·2026
Same author

Antiproliferative and Pro-Apoptotic Effects of <i>Tuber borchii</i> Extracts on Human Colorectal Cancer Cells via p53-Dependent Pathway Activation.

Metabolites·2025
Same author

Bioactive Compounds and Antioxidant Potential of Truffles: A Comprehensive Review.

Antioxidants (Basel, Switzerland)·2025

Related Experiment Video

Updated: Nov 15, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.3K

Current Methods for the Isolation of Urinary Extracellular Vesicles.

Serena Maggio1, Emanuela Polidori1, Paola Ceccaroli1

  • 1Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|March 2, 2021
PubMed
Summary

Extracellular vesicles (EVs), found in body fluids, show promise for diagnosing diseases. This chapter reviews methods for isolating urinary EVs (uEVs) to overcome challenges in their clinical use.

Keywords:
Density gradient separationExosomesImmunoprecipitationLiquid biopsySize-exclusion chromatographyUltracentrifugationUrine

More Related Videos

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.1K
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

4.4K

Related Experiment Videos

Last Updated: Nov 15, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.3K
Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.1K
Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

4.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Extracellular vesicles (EVs) are cell-secreted particles found in bodily fluids, carrying biomolecules for intercellular communication.
  • Urinary EVs (uEVs) reflect kidney and urinary tract health, offering diagnostic potential for kidney diseases.
  • Current EV isolation methods face challenges in purity and consistency, hindering clinical translation.

Purpose of the Study:

  • To review the advantages and disadvantages of various EV isolation techniques.
  • To provide practical examples of urinary EV (uEV) isolation protocols.
  • To guide the selection of appropriate methods based on time, cost, equipment, and downstream analysis needs.

Main Methods:

  • Comparative analysis of different EV isolation methodologies.
  • Description of uEV isolation protocols with varying resource requirements.
  • Consideration of sample input and downstream assay compatibility.

Main Results:

  • EVs are valuable biomarkers due to their biomolecule cargo.
  • uEVs offer a non-invasive window into kidney physiology and pathology.
  • Standardized, high-purity EV isolation remains a critical hurdle for clinical applications.

Conclusions:

  • Optimized EV isolation is crucial for realizing their diagnostic and therapeutic potential.
  • This chapter provides a guide to selecting suitable uEV isolation methods.
  • Addressing methodological limitations will accelerate the clinical translation of EV-based diagnostics.