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Updated: Nov 15, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Julia Álvarez1, José Manuel Fernández Real2, Francisco Guarner3
1Servicio de Endocrinología y Nutrición, Hospital Universitario Príncipe de Asturias, Meco, Madrid, España; Departamento de Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, España.
The human body hosts a diverse gut microbiota essential for health. Imbalances, or dysbiosis, linked to modern lifestyle, contribute to chronic diseases, highlighting the need to maintain microbial diversity.
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