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miRNA-296-5p functions as a potential tumor suppressor in human osteosarcoma by targeting SND1

Ya-Zeng Huang1, Jun Zhang2,3, Jian-Jian Shen4

  • 1Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Soochow, Jiangsu 215004, China.

Chinese Medical Journal
|March 2, 2021
PubMed
Abstract

Insights

MicroRNA (miR)-296-5p is downregulated in osteosarcoma (OS). Upregulating miR-296-5p inhibits OS cell proliferation, migration, and invasion by targeting staphylococcal nuclease and tudor domain containing 1 (SND1).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) pathogenesis remains unclear, necessitating novel therapeutic targets.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • This study investigates the role of miR-296-5p in OS.

Purpose of the Study:

  • To determine the expression levels of miR-296-5p in OS.
  • To elucidate the mechanism by which miR-296-5p exerts anti-OS effects.
  • To identify potential therapeutic targets for OS treatment.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure miR-296-5p expression in OS cell lines and tissues.
  • In vitro assays to assess the impact of miR-296-5p on OS cell proliferation, migration, and invasion.
  • Bioinformatic analysis and dual-luciferase reporter assays to identify and validate miR-296-5p targets.

Main Results:

  • miR-296-5p was significantly downregulated in OS cell lines and tissues compared to controls.
  • Overexpression of miR-296-5p suppressed proliferation, migration, and invasion of OS cells.
  • Staphylococcal nuclease and tudor domain containing 1 (SND1) was identified as a direct target of miR-296-5p, and its overexpression abrogated the anti-tumor effects of miR-296-5p.

Conclusions:

  • miR-296-5p acts as a tumor suppressor in osteosarcoma.
  • Targeting the miR-296-5p/SND1 axis presents a potential therapeutic strategy for OS.

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