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Sequence of a human kidney cDNA clone encoding thymosin beta 10.

V McCreary1, S Kartha, G I Bell

  • 1Department of Medicine, University of Chicago, Illinois 60637.

Biochemical and Biophysical Research Communications
|April 29, 1988
PubMed
Summary

Researchers identified thymosin beta 10 (Tβ10) in human kidney cells. This protein is highly conserved between humans and rats and its gene expression is regulated by serum stimulation in monkey kidney cells.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Thymosin beta 10 (Tβ10) is a protein involved in various cellular processes.
  • Understanding the genetic regulation of Tβ10 is crucial for comprehending its biological functions.

Purpose of the Study:

  • To isolate and characterize the human thymosin beta 10 cDNA.
  • To investigate the expression pattern and regulation of the thymosin beta 10 gene.

Main Methods:

  • cDNA library screening using a thymosin beta 10 probe.
  • RNA isolation and Northern blot analysis.
  • Sequence comparison between human and rat thymosin beta 10.

Main Results:

  • A cDNA clone encoding human thymosin beta 10 was successfully isolated.

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  • Human and rat thymosin beta 10 sequences show high conservation (100% amino acid, 95% nucleotide).
  • Thymosin beta 10 gene is constitutively expressed in monkey kidney cells, with a notable increase in a 2.4 kb transcript upon serum stimulation.

    • Conclusions:

    • Human thymosin beta 10 is a highly conserved protein.
    • The expression of thymosin beta 10 is regulated by serum in kidney epithelial cells, suggesting a role in cellular response to growth factors.