Related Experiment Video
Updated: Nov 15, 2025
10:26
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
Published on: November 7, 2019
5.8K
Label-free proteomics uncovers SMC1A expression is Down-regulated in AUB-E
Yingxian Jia1, Jie Luo1, Yibing Lan1
1Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.
Reproductive Biology and Endocrinology : RB&E
|March 3, 2021
Summary
Structural maintenance of chromosomes protein 1A (SMC1A) down-regulation may cause heavy menstrual bleeding in women with endometrial disorders. This finding offers a new understanding of abnormal uterine bleeding causes.
Area of Science:
- Gynecology
- Proteomics
- Cell Biology
Background:
- Heavy menstrual bleeding (HMB) is a common symptom in women with abnormal uterine bleeding caused by endometrial disorder (AUB-E).
- The underlying primary endometrial disorder responsible for AUB-E is not well understood.
Purpose of the Study:
- To investigate the proteomic differences in endometrial tissue between women with AUB-E and healthy controls.
- To identify potential molecular mechanisms contributing to AUB-E.
Main Methods:
- Comparative proteomic analysis using a label-free approach with LTQ-Orbitrap Elite mass spectrometry on endometrial samples.
- Validation of differentially expressed proteins using western blot and immunohistochemistry.
Main Results:
- Quantification of 2353 protein groups with a false discovery rate <1%.
- Identification of 291 significantly differentially expressed proteins between AUB-E patients and controls.
- Significant down-regulation of structural maintenance of chromosomes protein 1A (SMC1A) in endometrial glandular epithelial cells of AUB-E patients.
Conclusions:
- Down-expression of SMC1A is identified as a potential novel mechanism contributing to AUB-E.
- Reduced SMC1A may regulate cell cycle progression in endometrial glandular epithelium, leading to abnormal bleeding.
