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Related Concept Videos

Proteomics01:33

Proteomics

8.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.8K
Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Related Experiment Video

Updated: Nov 15, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

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Single-Cell Proteomics.

Luke F Vistain1, Savaş Tay1

  • 1Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, USA; Institute for Genomics and Systems Biology, University of Chicago, Chicago, IL 60637, USA.

Trends in Biochemical Sciences
|March 3, 2021
PubMed
Summary
This summary is machine-generated.

Measuring a cell's proteins (proteome) is difficult. Recent advances in microfluidics and encoding now allow hundreds of protein measurements, improving our understanding of gene expression and cellular function.

Keywords:
multiomicmultiplexingproteometargeted proteomicsuntargeted proteomics

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Area of Science:

  • Proteomics
  • Cell Biology
  • Biotechnology

Background:

  • Understanding gene expression requires measuring cellular proteins (proteome).
  • Current single-cell proteomic methods are limited, focusing on either few proteins or low multiplexing.
  • A bottleneck exists in making broad, unbiased proteome measurements.

Purpose of the Study:

  • To review recent advances in single-cell proteomic measurement technologies.
  • To highlight progress enabling hundreds of protein measurements and high-sensitivity quantification.
  • To discuss emerging technologies for unbiased cellular proteome analysis.

Main Methods:

  • Review of microfluidics and output encoding advancements.
  • Analysis of technologies for high-multiplex and ultrahigh-sensitivity protein quantification.
  • Exploration of emerging single-cell mass spectrometry.

Main Results:

  • Significant improvements in single-cell proteomic measurement capabilities.
  • Enabling of hundreds of protein measurements per cell.
  • Advancements in ultrahigh-sensitivity protein quantification.

Conclusions:

  • Recent technological progress addresses limitations in single-cell proteomic analysis.
  • Emerging technologies promise more comprehensive and unbiased proteome measurements.
  • Improved proteomic insights will advance understanding of gene expression and cellular phenotype.