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The effect of dietary lipid hydroperoxide on lymphoid tissues in mice
1Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Abstract:
Effects of dietary lipid hydroperoxides on lymphoid tissue were studied in mice. When graded amounts (190, 270 and 310 mg) of methyl linoleate hydroperoxide (MLHPO) were orally administered to male C57BL/6 mice (6 weeks old), necrosis was observed in lymphocytes located among the reticular network in the thymus, and thymus weight was significantly decreased 24 h after the treatment. The spleen weight of mice given MLHPO tended to decrease. Spontaneous chemiluminescence of the thymus was remarkably increased after the dose. Thiobarbituric acid reactants in the liver, thymus and blood were also increased after the dose of MLHPO. At intervals of 3, 6, 12 and 24 h after a dose of 14C-labeled MLHPO, 14C was detected in the blood and liver. Fatty infiltration of the liver was found after the treatment with MLHPO. These findings indicate that oral intake of lipid hydroperoxides causes significant damage to lymphoid tissues of mice.
Insights
Dietary lipid hydroperoxides, such as methyl linoleate hydroperoxide (MLHPO), damage mouse lymphoid tissues. Oral MLHPO intake caused thymus necrosis, decreased thymus and spleen weight, and increased oxidative stress markers.
Area of Science:
- Immunology
- Toxicology
- Biochemistry
Background:
- Dietary lipids can form hydroperoxides, which are reactive oxygen species.
- The impact of ingested lipid hydroperoxides on lymphoid tissues is not fully understood.
Purpose of the Study:
- To investigate the effects of oral administration of methyl linoleate hydroperoxide (MLHPO) on mouse lymphoid tissues.
- To assess the systemic distribution and biochemical changes induced by MLHPO.
Main Methods:
- Male C57BL/6 mice were orally administered graded doses of MLHPO.
- Thymus and spleen weights were measured.
- Spontaneous chemiluminescence and thiobarbituric acid reactants were assessed in various tissues.
- 14C-labeled MLHPO was used to track its distribution.
- Histopathological examination of the liver was performed.
Main Results:
- MLHPO administration led to thymus necrosis and significantly decreased thymus weight.
- Spleen weight showed a tendency to decrease.
- Increased spontaneous chemiluminescence and thiobarbituric acid reactants were observed in the thymus, liver, and blood.
- 14C-MLHPO was detected in blood and liver, with evidence of fatty infiltration in the liver.
Conclusions:
- Oral intake of lipid hydroperoxides causes significant damage to mouse lymphoid tissues.
- MLHPO exposure induces oxidative stress and histopathological changes in the liver.