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Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: Nov 15, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Near-infrared temperature-switchable fluorescence nanoparticles.

Shuai Yu^1,2, Zhen Wang^1,2, Tingfeng Yao^1,2

¹Ultrasound and Optical Imaging Laboratory, Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA.

Quantitative Imaging in Medicine and Surgery

|March 3, 2021

Summary

This summary is machine-generated.

New near-infrared (NIR) nanoparticles exhibit switchable fluorescence for temperature sensing. Researchers controlled nanoparticle properties to enable precise in vivo temperature imaging and biomedical applications.

Keywords:

Aza-BODIPY and Zinc phthalocyanine fluorophores Near infrared temperature-switchable fluorescence imaging and sensing pluronic nanoparticles

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Published on: March 17, 2023

Area of Science:

Biomedical Engineering
Materials Science
Nanotechnology

Background:

Near-infrared (NIR) fluorophores are crucial for biomedical applications due to their non-invasive nature, sensitivity, and deep tissue penetration.
Encapsulating NIR fluorophores in thermal-sensitive polymers creates effective temperature-sensing probes.

Purpose of the Study:

To synthesize and characterize NIR temperature-switchable nanoparticles.
To investigate factors influencing temperature thresholds (T_th) for controlled synthesis.

Main Methods:

Synthesized nanoparticles using aza-BODIPY (ADP) or Zinc phthalocyanine (ZnPc) fluorophores and Pluronic polymers (F127, F98, F68, F38).
Utilized sonication for fluorophore encapsulation, creating nanoparticles with switch-like fluorescence responses to temperature.
Examined effects of pluronic type, nanoparticle concentration, and fluorophore type on T_th.

Main Results:

Demonstrated excellent temperature-switchable fluorescence intensity and/or lifetime properties.
Identified pluronic category and nanoparticle concentration as significant factors affecting T_th.
Observed that fluorophore category had a weaker effect on T_th.

Conclusions:

Achieved tunable temperature thresholds (T_th) by selecting Pluronic polymers and adjusting nanoparticle concentration.
Developed temperature-switchable fluorescence nanoparticles suitable for biomedical imaging.
Enabled in vivo tissue temperature sensing and imaging applications.