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Three-dimensional Optical-resolution Photoacoustic Microscopy
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Live feedback and 3D photoacoustic remote sensing.

Saad Abbasi1, Kevan Bell1,2, Benjamin Ecclestone1

  • 1PhotoMedicine Labs, Department of Systems Design Engineering, University of Waterloo, Waterloo, Ontario, Canada.

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|March 3, 2021
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Summary

This study introduces a novel photoacoustic remote sensing (PARS) method for high-resolution, real-time imaging. The technique achieves video-rate live feedback, crucial for clinical applications, with improved resolution and sensitivity.

Keywords:
3D imagingPhotoacoustic (PA)live-feedbackmicroscopyremotesensing

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Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Microscopy

Background:

  • Clinical adoption of photoacoustic (PA) techniques requires high-speed live feedback.
  • Conventional PA microscopy faces challenges in balancing resolution, sensitivity, and speed due to opto-acoustic confocal geometry.
  • Photoacoustic Remote Sensing (PARS) offers an all-optical solution for high-resolution live PA imaging.

Purpose of the Study:

  • To develop a high-resolution, real-time live display system for reflection-mode photoacoustic microscopy.
  • To overcome the limitations of conventional PA techniques in achieving video-rate imaging.
  • To demonstrate the feasibility of 3D imaging using a non-contact, label-free PARS approach.

Main Methods:

  • Implementation of a 2D galvanometer scanner system with a 600 KHz pulse repetition rate laser for 2.5 frames per second acquisition.
  • Development of a computationally inexpensive image reconstruction algorithm for real-time frame rendering.
  • Utilizing photoacoustic remote sensing (PARS) with an all-optical confocal geometry.

Main Results:

  • Achieved live feedback frame rates of 2.5 Hz in 2D with 1.2 µm resolution, the highest for real-time reflection-mode PA.
  • Demonstrated the first 3D imaging capabilities with a non-contact, label-free reflection-mode PARS technique.
  • Validated the system with phantom studies and in-vivo imaging, showing high signal-to-noise ratios (44 dB mean, 62 dB peak).

Conclusions:

  • The developed PARS method enables real-time, high-resolution, and sensitive reflection-mode photoacoustic imaging with minimal computational overhead.
  • The study presents the first 3D imaging results using a non-contact, label-free PARS technique.
  • This work is a significant advancement towards clinical translation of video-rate, high-resolution reflection-mode PA imaging systems.