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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Nov 15, 2025

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

Ribonucleoprotein Immunoprecipitation (RIP) Analysis.

Jennifer L Martindale¹, Myriam Gorospe¹, Maria L Idda¹

¹Laboratory of Genetics and Genomics, Biomedical Research Center, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, USA.

|March 3, 2021

Summary

This summary is machine-generated.

RNP immunoprecipitation (RIP) analysis rapidly identifies endogenous RNAs bound to RNA-binding proteins (RBPs). This cost-effective method monitors RNA-protein interactions under various conditions, aiding gene expression studies.

Keywords:

Gene expression Post-transcriptional regulation RNA target RNA-binding proteins Ribonucleoprotein complex

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Published on: September 29, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Related Experiment Videos

Last Updated: Nov 15, 2025

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Ribonucleoprotein (RNP) complexes, formed by RNAs and RNA-binding proteins (RBPs), are crucial for regulating gene expression.
Investigating dynamic RNA-RBP associations is essential for understanding gene regulation.
Native RNP immunoprecipitation (RIP) is a key technique for studying these interactions.

Purpose of the Study:

To provide an optimized protocol for RNP immunoprecipitation (RIP) analysis.
To enable the identification of endogenous RNAs associated with specific RBPs.
To facilitate the study of RNA-protein interactions in various biological contexts.

Main Methods:

Utilizes an antibody, typically targeting an RBP, to immunoprecipitate native RNP complexes.
Isolates RNA from the immunoprecipitated complexes.
Employs downstream analyses such as PCR, microarray, Northern blot, and sequencing.

Main Results:

Enables rapid identification of endogenous RNAs bound to RBPs.
Monitors time-dependent and condition-specific changes in RNA-RBP associations.
Offers a cost-effective method for analyzing RNP interactions across multiple samples.

Conclusions:

Optimized RIP protocol provides a versatile tool for studying RNA-protein interactions.
Facilitates research in diverse areas of biology by characterizing RNP complex dynamics.
Addresses limitations of traditional RIP by enabling the study of endogenous RNA-RBP associations.