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RNA Sequencing of Single Myofibers from Mus musculus
Darren M Blackburn1,2, Felicia Lazure1,2, Vahab D Soleimani1,2
1Department of Human Genetics, McGill University, 3640 rue University, Montreal, QC, H3A 0C7 Canada.
Abstract:
Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the transcriptome of myofibers we combine single myofiber isolation with SMART-Seq to provide high resolution genome wide expression of a single myofiber.
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