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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
Published on: November 11, 2010
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Neurostore: A Novel Cryopreserving Medium for Primary Neurons
Francesca Pischedda1, Giovanni Piccoli1
1CIBIO, Dulbecco Telethon Institute, University of Trento, Trento, Italy.
Bio-Protocol
|March 3, 2021
Summary
Cryopreservation of primary neurons using Neurostore buffer is now possible. This method reduces animal sacrifice and waste, offering a valuable resource for in vitro neurobiology research.
Area of Science:
- Neurobiology
- Cell Biology
- Cryobiology
Background:
- Primary neuronal cultures are essential for neurobiology research.
- Current methods require extensive planning and result in significant cell waste.
- Animal sacrifice is a concern in primary cell culture preparation.
Purpose of the Study:
- To evaluate the efficacy of Neurostore buffer for cryopreservation of primary neurons.
- To provide a method for reducing animal use and cell waste in neurobiology research.
Main Methods:
- Primary neuronal cells were cultured from rodents.
- Cryopreservation was performed using Neurostore buffer.
- Cell viability and function post-thaw were assessed.
Main Results:
- Neurostore buffer successfully supported cryopreservation of primary neurons.
- Cryopreserved neurons retained viability and function after thawing.
- This method offers a viable alternative to fresh primary cultures.
Conclusions:
- Neurostore buffer is an effective medium for cryopreserving primary neurons.
- Cryopreservation reduces the need for repeated animal procedures and cell waste.
- This technique enhances the utility of primary neuronal cultures for in vitro studies.
