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Protocol for Measuring Compulsive-like Feeding Behavior in Mice.

Marta Fructuoso1,2, Jose Espinosa-Carrasco2,3, Ionas Erb2,3

  • 1Cellular and Systems Neurobiology, Systems Biology Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), Dr. Aiguader 88, Barcelona 08003, Spain.

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Summary
This summary is machine-generated.

This study presents a new mouse model protocol to study overeating and diet-induced obesity (DIO). It allows for the longitudinal characterization of feeding behaviors and the detection of abnormal eating patterns in mice.

Keywords:
BehaviorBehavioral data visualizationCompulsive overeatingComputational behavioral neuroscienceEnergy dense foodsLongitudinal behavioral analysisMouse model

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Area of Science:

  • Neuroscience
  • Behavioral Science
  • Obesity Research

Background:

  • Obesity is a global health crisis driven by environmental factors and high-caloric food availability.
  • Understanding the primary dysfunctions in feeding behavior, not just the outcome of obesity, is crucial for developing effective therapies.
  • Existing animal models often focus on obesity outcomes rather than the underlying behavioral changes in palatable food consumption.

Purpose of the Study:

  • To describe a novel protocol for studying overeating in mice using diet-induced obesity (DIO).
  • To enable the longitudinal characterization of feeding behavior and detect abnormal eating patterns.
  • To validate the use of animal models for investigating the non-homeostatic factors driving overeating.

Main Methods:

  • Utilizing a diet-induced obesity (DIO) protocol in mice with free-choice or forced intake of palatable/calorie-dense diets.
  • Longitudinal registration and analysis of meal patterns during the weight gain period.
  • Incorporating behavioral tests, including challenges with bitter taste (quinine) and limited food access, to assess compulsive feeding and food preferences.
  • Computational analysis of large longitudinal behavioral datasets.

Main Results:

  • The protocol successfully induces changes in meal patterns indicative of overeating in mice.
  • Abnormal eating behaviors, such as binge eating and snacking, were detected, mirroring human conditions.
  • Mice demonstrated a preference for palatable foods, highlighting their reinforcing effects.
  • Compulsive feeding components were observed, including continued consumption despite adverse taste and neglect of standard chow.

Conclusions:

  • The described DIO protocol provides a robust method for studying overeating and associated behavioral changes in mice.
  • This model allows for the longitudinal characterization of feeding behavior, aiding in the understanding of non-homeostatic food intake.
  • The protocol facilitates the identification of compulsive overeating and supports the development of novel therapeutic strategies for obesity.