Production of Quantum Dots-containing Influenza Virus Particles for Studying Viral Uncoating Processes

The genome of influenza A virus (IAV) comprises eight pinlike genomic segments called vRNPs enclosed in viral capsid. During infection, uncoating is the key step for viral replication and represents an antiviral therapeutic target, but it is difficult to observe the transient and dynamic event in detail. Here, we report a protocol for production of quantum dots-containing influenza virus particles by encapsulating quantum dot-conjugated vRNPs during viral assembly. These labeled virions can be used for monitoring viral trafficking in real time and studying viral uncoating processes.