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Updated: Nov 15, 2025

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
Published on: February 7, 2011
Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal
Daniel J Fazakerley1, Andreas M Fritzen1,2, Marin E Nelson1
1Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia.
Abstract:
Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously.
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