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Related Experiment Video

Updated: Nov 15, 2025

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

925

Platelet Isolation and Activation Assays.

Laura C Burzynski1, Nicholas Pugh2, Murray C H Clarke1

  • 1Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK.

Bio-Protocol
|March 3, 2021
PubMed
Summary
This summary is machine-generated.

This study details methods for isolating, counting, and activating platelets. It enables rapid quantification of platelet responses using flow cytometry for better understanding of thrombosis and hemostasis.

Keywords:
Flow cytometryPlatelet activationPlatelet enumerationPlatelet isolationPlatelets

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Area of Science:

  • Hematology
  • Immunology
  • Biochemistry

Background:

  • Platelets are crucial for hemostasis and pathogenic thrombosis after atherosclerotic plaque rupture.
  • Platelet activation, triggered by endothelial damage, involves granule release, receptor activation, and phosphatidylserine externalization.
  • These activation processes are fundamental to thrombus formation and contribute to pathological conditions.

Purpose of the Study:

  • To present protocols for platelet isolation, counting, and activation.
  • To enable rapid quantification of platelet-associated responses, particularly cell surface protein expression.
  • To provide tools for studying platelet function in hemostasis and thrombosis.

Main Methods:

  • Isolation and counting of platelets from blood samples.
  • Controlled activation of platelets under experimental conditions.
  • Flow cytometry for rapid quantification of cell surface protein expression on activated platelets.

Main Results:

  • Established protocols for reliable platelet isolation and activation.
  • Demonstrated the utility of flow cytometry for quantifying platelet responses.
  • Quantified changes in cell surface proteins indicative of platelet activation.

Conclusions:

  • Accurate quantification of platelet responses is vital for understanding hemostasis and thrombosis.
  • The described flow cytometry protocols offer a rapid and effective method for assessing platelet activation.
  • These methods can advance research into platelet-related diseases and therapeutic strategies.