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Updated: Nov 15, 2025
Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
Published on: June 23, 2023
Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains
1Developmental Neurobiology Department, Instituto de Neurociencias-Consejo Superior de Investigaciones Científicas/Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Alicante, Spain.
Abstract:
The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells within the Outer Proliferation Center (OPC) of Drosophila larval brains.
