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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Related Experiment Video

Updated: Nov 15, 2025

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Preparing Single-cell DNA Library Using Nextera for Detection of CNV.

Larry Xi¹, Patrick Leong¹, Aleksandar Mihajlovic¹

¹Digenomix Corp, South San Francisco, CA 94080, USA.

|March 3, 2021

Summary

Single-cell DNA sequencing offers quantitative insights into tissue heterogeneity, outperforming bulk sequencing. The Transposon Barcoded (TnBC) method provides a simple, versatile approach for single-cell library preparation and copy number variation analysis.

Keywords:

Copy number variation Nextera Shallow sequencing Single-cell Transposon

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Last Updated: Nov 15, 2025

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Area of Science:

Genomics and Molecular Biology
Cancer Research
Biotechnology

Background:

Heterogeneous tissues, such as tumors, present complex biological questions that bulk sequencing cannot fully address.
Single-cell DNA sequencing enables a quantitative evaluation of cellular heterogeneity.
Several single-cell library preparation methods exist, including DOP-PCR, MDA, MALBAC, LIANTI, and TnBC.

Purpose of the Study:

To highlight the utility of single-cell DNA sequencing for analyzing tissue heterogeneity.
To introduce and emphasize the Transposon Barcoded (TnBC) library preparation method.
To showcase TnBC's capabilities in detecting copy number variations and quantifying gene copy numbers per cell.

Main Methods:

Review of existing single-cell DNA library preparation techniques.
Focus on the Transposon Barcoded (TnBC) methodology.
Application of TnBC for copy number variation detection and absolute gene copy number determination.

Main Results:

Single-cell DNA sequencing provides a resolution unattainable by bulk sequencing for heterogeneous samples.
The TnBC method is presented as a simple and versatile option for single-cell library preparation.
TnBC facilitates the accurate detection of copy number variations and the quantification of gene copy numbers at the single-cell level.

Conclusions:

Single-cell DNA sequencing is crucial for understanding cancer heterogeneity.
The TnBC library preparation method is an effective tool for single-cell genomic analysis.
TnBC enables precise copy number profiling of individual cells.