Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 15, 2025

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
09:30

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Published on: October 16, 2019

7.2K

Acute Cerebellar Slice Preparation Using a Tissue Chopper.

Yubin Wang1, Michel Baudry1

  • 1Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA.

Bio-Protocol
|March 3, 2021
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calpain-2 Inhibition or Deletion Enhances Levels of the Transcription Factor, MEIS2, and Stimulates Neurogenesis.

Current neuropharmacology·2026
Same author

Lymphocyte variant hypereosinophilic syndrome with severe eosinophilic pneumonia treated with mepolizumab: a case report.

Central-European journal of immunology·2026
Same author

Visible-Light-Catalyzed Tandem Borylation/Cyclization of 1,7-Enynes with NHC-Boranes for the Synthesis of Boron-Embedded Polycyclic 3,4-Dihydroquinolin-2(1<i>H</i>)-ones.

The Journal of organic chemistry·2026
Same author

Association between duration of neurosurgical craniotomy and surgical site infection: a prospective cohort study.

The Journal of hospital infection·2026
Same author

New Insights into the Complexation of Metal Ions with Xanthate in the Flotation Process of Sulfide Minerals: A Study Based on Pyrite.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Multi-layer causal knowledge network for product quality problem analysis using third data.

Scientific reports·2026
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
See all related articles

A simpler method for preparing acute cerebellar slices using a tissue chopper is presented. This faster, easier technique yields versatile slices for neuroscience research in rodents.

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Physiology

Background:

  • Acute cerebellar slices are crucial for studying synaptic transmission and intracellular signaling in the cerebellum.
  • Existing methods, often using vibrating microtomes, can be complex.
  • The cerebellar cortex offers a well-organized structure for reliable neuronal pathway studies.

Purpose of the Study:

  • To introduce a simpler, faster method for preparing acute sagittal cerebellar slices from rodents.
  • To provide an alternative to existing, more complex slice preparation techniques.
  • To enhance the versatility of cerebellar slice applications in neuroscience.

Main Methods:

  • Utilizing a tissue chopper for slicing rodent cerebellum into 200-400 µm thick sections.
Keywords:
Acute cerebellar sliceCerebellumElectrophysiologyMouseRat

More Related Videos

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
09:41

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Published on: March 20, 2019

11.4K
Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures
06:31

Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Published on: December 18, 2019

8.4K

Related Experiment Videos

Last Updated: Nov 15, 2025

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
09:30

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Published on: October 16, 2019

7.2K
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
09:41

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Published on: March 20, 2019

11.4K
Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures
06:31

Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Published on: December 18, 2019

8.4K
  • Dissecting cerebellum from the whole brain.
  • Allowing slices to recover in oxygenated artificial cerebrospinal fluid (aCSF) at 37°C for 1-2 hours.

    • Main Results:

    • The tissue chopper method is faster and easier than traditional vibrating microtome techniques.
    • The method is applicable to both mice and rats across a wide age range (postnatal day 7 to 2 years).
    • The prepared slices are suitable for various experimental applications, including electrophysiology.

    Conclusions:

    • This tissue chopper method offers a more accessible and versatile approach to acute cerebellar slice preparation.
    • The technique simplifies research on cerebellar function, synaptic transmission, and signaling pathways.
    • This method expands the utility of acute cerebellar slices for a broader range of neuroscience investigations.