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Updated: Nov 15, 2025
Optical Clearing and Imaging of Immunolabeled Kidney Tissue
Published on: July 22, 2019
Immunofluorescence staining of WT-1/Podocalyxin on Mouse kidney sections
Carole Henique1, Olivia Lenoir2, Pierre-Louis Tharaux2,3
1Institut Mondor de Recherche Biomédicale (IMRB), Inserm U955, Université Paris Est Créteil UPEC, Equipe 21, Créteil, France.
In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes with a specific marker WT-1 (Wilms Tumor-1) for "in situ" podocyte analysis by immunofluorescent microscopy, more informative technique than other techniques (as Western blot).
In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes with a specific marker WT-1 (Wilms Tumor-1) for "in situ" podocyte analysis by immunofluorescent microscopy, more informative technique than other techniques (as Western blot).
