Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.1K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.1K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.6K
Proteomics01:33

Proteomics

8.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.8K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.3K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Combined Omics Approach to Elucidate the Molecular Interplay behind a Beneficial <i>Arabidopsis-Caulobacter</i> Interaction.

Journal of proteome research·2026
Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same author

Single-cell proteomics reveals cytoplasmic defects in Patl2-deficient oocytes rescued by spindle transfer.

Human reproduction (Oxford, England)·2026
Same author

Advancing DIA-Based Limited Proteolysis Workflows: Introducing DIA-LiPA.

Analytical chemistry·2026
Same author

Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same author

Structural and Proteomic Analysis of the Mouse Cathepsin B-DARPin 4m3 Complex Reveals Species-Specific Binding Determinants.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Nov 15, 2025

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

2.8K

Mass spectrometry and the cellular surfaceome.

Jarne Pauwels1,2, Daria Fijałkowska1, Sven Eyckerman1,2

  • 1VIB Center for Medical Biotechnology, VIB, Ghent, Belgium.

Mass Spectrometry Reviews
|March 3, 2021
PubMed
Summary

This review explores methods for analyzing the surfaceome, the collection of exposed plasma membrane proteins. It addresses challenges in mass spectrometry-based proteomics for studying these vital cell surface proteins.

Keywords:
LC-MS/MSenrichment strategiesplasma membrane proteinsproteomicssurfaceome

More Related Videos

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

11.5K
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

11.0K

Related Experiment Videos

Last Updated: Nov 15, 2025

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

2.8K
Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

11.5K
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
15:00

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

11.0K

Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • The surfaceome, comprising exposed plasma membrane proteins, is crucial for cellular processes like communication and transport.
  • Surface proteins are vital in immunity, cell signaling, and disease pathogenesis, making them key targets for biomarkers and therapeutics.
  • Analyzing the surfaceome presents challenges due to inefficient protein extraction and low abundance, often leading to underrepresentation in proteomic data.

Purpose of the Study:

  • To provide a comprehensive overview of current methodologies for surfaceome analysis.
  • To discuss the specific considerations for downstream mass spectrometry-based proteomics of surface proteins.
  • To highlight innovative analytical approaches developed to overcome existing challenges in surfaceome research.

Main Methods:

  • Review of existing literature on surfaceome isolation and enrichment techniques.
  • Analysis of various strategies for enhancing the extraction and detection of membrane proteins.
  • Discussion of mass spectrometry-based proteomics workflows tailored for surfaceome studies.

Main Results:

  • Identification of diverse methods for enriching and analyzing the surfaceome.
  • Evaluation of techniques addressing the challenges of hydrophobic protein extraction and low abundance.
  • Consideration of downstream mass spectrometry applications for comprehensive surfaceome profiling.

Conclusions:

  • Despite analytical challenges, advancements in methodologies are improving surfaceome characterization.
  • Effective surfaceome analysis is critical for identifying novel biomarkers and therapeutic targets.
  • This review offers insights into state-of-the-art techniques for advancing surfaceome research through proteomics.