The morphology and distribution of rat serotoninergic intraspinal neurons: an immunohistochemical study

An immunohistochemically derived morphological description of a diverse population of rat lamina VII and X intraspinal 5HT neurons is provided. These bipolar or multipolar neurons occur most frequently in lamina X, dorsal or dorsolateral to the central canal, in thoracolumbar, sacral, and coccygeal spinal segments. These 5HT intraspinal neurons are found in normal rat spinal cords as well as in spinal cords that have been hemisected or transected 60 days prior to serotonin immunostaining. Therefore, 5HT intraspinal neurons are the probable source of the biochemically detectable 5HT that remains in the spinal cord distal to a spinal transection. In the rat, serotonin intraspinal neurons are most often associated with spinal autonomic nuclei but it is unknown if they are preganglionic in nature.