β-Galactosidase: From its source and applications to its recombinant form

Area of Science:

• Biochemistry
• Industrial Biotechnology

Background:

• Carbohydrate-active enzymes, including glycosidases, are vital for carbohydrate metabolism.
• Beta-galactosidase (β-galactosidase) is a key β-glycosidase found across various organisms.
• It plays a significant role in the food industry for lactose hydrolysis.

Purpose of the Study:

• To review the origins, structure, and recombinant production of β-galactosidase.
• To discuss critical modifications for enhancing β-galactosidase production processes.
• To highlight the enzyme's importance in industrial biotechnology and applied biochemistry.

Main Methods:

• Literature review of β-galactosidase research.
• Analysis of recombinant production techniques.
• Examination of enzyme modification strategies.

Main Results:

• β-Galactosidase is essential for producing lactose-free dairy products with enhanced sweetness and digestibility.
• Recombinant technology offers a valuable method for producing this enzyme.
• Modifications can optimize production and application.

Conclusions:

• β-Galactosidase is a valuable enzyme with diverse applications in the food industry and healthcare.
• Understanding its properties and production is crucial for industrial biotechnology.
• Further research into enzyme modifications can improve its industrial utility.