Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody
Xiangguang Lyu1, Hongyu Li1, Bin Liu2
1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Abstract:
Cryptochromes (CRYs) belong to an ancient and conserved class of blue-light receptor regulating circadian clock and development in animals and plants. Arabidopsis CRY2 form physiologically active homodimers in response to blue light treatment and further oligomerize into photobodies, which are expected to be the foci harboring protein interaction, phosphorylation, and ubiquitination. Here we describe two efficient methods developed to test the formation of blue-light-dependent photobodies of CRY-GFP fusing proteins using the mesophyll protoplasts of Arabidopsis or soybean, respectively.
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