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Updated: Nov 15, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis
Tian-Ping Chen1, Nai-Ju Zhang2, Hong-Ju Wang2
1Department of Cardiology, the First Affiliated Hospital of Bengbu Medical College, No. 287, Changhuai Road, Longzi Lake District, Bengbu city, 233003, Anhui province, China. ra3330@163.com.
Insights
Circular RNA (circRNA) circROBO2 is upregulated in acute myocardial infarction (AMI) and exacerbates cardiac injury by sponging miR-1184 and increasing TRADD. Knockdown of circROBO2 protects the heart by restoring miR-1184 levels and reducing TRADD.
Area of Science:
- Cardiovascular Biology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are implicated in cardiovascular diseases.
- The specific role of circROBO2 in acute myocardial infarction (AMI) remains largely unknown.
- This study investigates the pathogenic involvement of circROBO2 in AMI.
Purpose of the Study:
- To elucidate the role and mechanism of circROBO2 in the pathogenesis of acute myocardial infarction (AMI).
- To determine the regulatory relationship between circROBO2, miR-1184, and TRADD in the context of myocardial injury.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) and Western blotting were employed to assess gene and protein expression.
- RNA immunoprecipitation (RIP) and luciferase reporter assays were used to validate molecular interactions.
- Flow cytometry and echocardiography were utilized to evaluate myocardial cell apoptosis and cardiac function.
Main Results:
- circROBO2 and TRADD expression were significantly elevated, while miR-1184 was downregulated in AMI models.
- circROBO2 functions as a molecular sponge for miR-1184, leading to increased TRADD expression.
- Overexpression of miR-1184 mitigated the effects of circROBO2 knockdown, partially inhibiting TRADD.
Conclusions:
- Knockdown of circROBO2 ameliorates myocardial injury post-AMI.
- This protective effect is mediated by the circROBO2/miR-1184/TRADD axis, reducing cardiomyocyte apoptosis.
- circROBO2 represents a potential therapeutic target for acute myocardial infarction.
Background:
Studies have found that circular RNAs (circRNAs) play key roles in cardiovascular diseases. However, the function of circROBO2 in acute myocardial infarction (AMI) is unclear. This study aimed to investigate the pathogenesis of circROBO2 in AMI.
Methods:
qRT-PCR and Western blot were used to determine the expression levels of circROBO2, miR-1184, and TRADD in AMI and sham-operated mouse models at mRNA and protein level, respectively. The relationship among miR-1184, circROBO2 and TRADD was evaluated by RNA immunoprecipitation (RIP) analysis and luciferase reporter gene analysis. The roles of circROBO2, miR-1184, and TRADD in myocardial cell apoptosis were evaluated using flow cytometry. Ultrasound echocardiography, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarction area, and myocardial cell apoptosis were measured to examine the effects of circROBO2 on myocardial injury.
Results:
The expression levels of miR-1184 were significantly reduced, and the expression levels of circROBO2 and TRADD were significantly increased in MI group. CircROBO2 acted as a sponge for miR-1184 by upregulating the expression of TRADD. In addition, overexpression of miR-1184 enhanced the protective effect of knockdown of circROBO2 by partially inhibiting the expression of TRADD in vivo and in vitro.
Conclusion:
Knockdown of circROBO2 reduced the apoptosis of cardiomyocytes by increasing the expression levels of miR-1184, which in turn decreased the expression levels of TRADD in the myocardium post-MI.
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