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Related Experiment Video

Updated: Nov 15, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
11:13

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

50.9K

Mouse Adipose Tissue Protein Extraction.

Yu A An1, Philipp E Scherer1

  • 1Touchstone Diabetes Center, Department of Internal Medicine; Department of Cell Biology, The University of Texas Southwestern Medical Center, Dallas, USA.

Bio-Protocol
|March 4, 2021
PubMed
Summary
This summary is machine-generated.

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This study presents an efficient, cost-effective protocol for extracting proteins from adipose tissue (AT). The new method saves time and resources, crucial for metabolism research on obesity.

Area of Science:

  • Metabolism and Endocrinology
  • Biochemistry
  • Molecular Biology

Background:

  • Obesity is a global epidemic, increasing focus on adipose tissue (AT) roles.
  • Extracting proteins from AT is difficult due to high lipid content.
  • Existing AT protein extraction methods are costly or time-consuming.

Purpose of the Study:

  • To develop an efficient, economical, and time-saving protocol for mouse adipose tissue protein extraction.
  • To provide a reliable method for researchers studying the physiological and pathological roles of AT.

Main Methods:

  • A detailed laboratory protocol for mouse adipose tissue protein extraction.
  • Utilizes common laboratory reagents and instruments.
  • Protocol completion time: 90-120 minutes.
Keywords:
Adipose TissueLipid ContaminationMetabolismMouseObesityProteinTime and Cost Effective

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Related Experiment Videos

Last Updated: Nov 15, 2025

Mouse Adipose Tissue Collection and Processing for RNA Analysis
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Mouse Adipose Tissue Collection and Processing for RNA Analysis

Published on: January 31, 2018

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Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
07:03

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Published on: June 12, 2020

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Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
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Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

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Main Results:

  • Achieves good recovery of total protein content from adipose tissue.
  • Requires only common reagents and instruments.
  • Offers a significant time and cost saving compared to existing methods.

Conclusions:

  • The described protocol is an economically attractive, time-saving, and efficient method for AT protein extraction.
  • This protocol supports ongoing metabolism research into obesity and adipose tissue function.
  • Facilitates protein analysis in adipose tissue for broader scientific application.