Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resorantel: a dual-targeting therapeutic with potent efficacy against Staphylococcus aureus with low potential for drug resistance.

Communications biology·2026
Same author

Drug repurposing: a dual-mechanism antibiotic combats MRSA and its high resistant phenotypes.

Frontiers in cellular and infection microbiology·2026
Same author

[Antibacterial activity and mechanism of the antiparasitic drug resorantel against <i>Staphylococcus epidermidis</i>].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2026
Same author

Communicating Confidence in the Reliability of Micro- and Nanoplastic Identification in Human Health Studies.

Environment & health (Washington, D.C.)·2026
Same author

Ubiquitination mediated cisplatin resistance in bladder cancer.

Anti-cancer drugs·2026
Same author

Mapping the miRNA landscape of primitive macrophage extracellular vesicles highlights their pro-vasculogenic effects in engineered human cardiac tissue.

APL bioengineering·2026

Related Experiment Video

Updated: Nov 15, 2025

Contrast Enhanced Vessel Imaging using MicroCT
05:50

Contrast Enhanced Vessel Imaging using MicroCT

Published on: January 27, 2011

12.9K

Extracellular Vesicles Tracking and Quantification Using CT and Optical Imaging in Rats.

Shaowei Guo1,2, Oshra Betzer3, Nisim Perets4

  • 1Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.

Bio-Protocol
|March 4, 2021
PubMed
Summary

This study presents a novel method for tracking and quantifying exosomes in the central nervous system following spinal cord injury. The protocol enables detailed analysis of exosome biodistribution at cellular and organ levels.

Keywords:
BiodistributionExosomesExtracellular vesiclesIn vivo imagingSpinal cord injuryTracking

More Related Videos

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
09:01

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Published on: May 23, 2021

3.9K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

8.6K

Related Experiment Videos

Last Updated: Nov 15, 2025

Contrast Enhanced Vessel Imaging using MicroCT
05:50

Contrast Enhanced Vessel Imaging using MicroCT

Published on: January 27, 2011

12.9K
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
09:01

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Published on: May 23, 2021

3.9K
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

8.6K

Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Cell Biology

Background:

  • Exosomes are crucial for intercellular communication, carrying proteins, lipids, and genetic material.
  • Understanding exosome biodistribution is vital for studying their biological actions and therapeutic potential.
  • Existing tracking methods for exosomes in vivo have limitations.

Purpose of the Study:

  • To develop and present a comprehensive protocol for tracking and quantifying exosomes in a spinal cord injury model.
  • To investigate the biodistribution of intranasally administered exosomes in the central nervous system of rats with spinal cord injury.
  • To assess the abundance and cellular affinity of exosomes in spinal cord lesions.

Main Methods:

  • Intranasal administration of gold nanoparticle-encapsulated exosomes in a rat spinal cord injury model.
  • Microcomputed tomography (microCT) for whole central nervous system exosome tracking.
  • Inductively coupled plasma and flame atomic absorption spectroscopy for exosome quantification.
  • Optical imaging of fluorescently labeled exosomes to determine lesion abundance and cell type affinity.

Main Results:

  • Successfully tracked and quantified exosomes throughout the central nervous system after intranasal administration.
  • Determined the abundance of migrating exosomes in spinal cord lesions compared to healthy controls.
  • Examined the affinity of exosomes to specific cell types within the spinal cord lesion.

Conclusions:

  • The presented protocol effectively aids in studying exosome biodistribution in spinal cord injury models at both cellular and organ levels.
  • This method provides valuable insights into the fate of administered exosomes.
  • The protocol can be adapted for studying exosome behavior in other disease models.