Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Filling the research gaps of malaria pathobiology.

Frontiers in microbiology·2026
Same author

First continuous in vitro culture of Plasmodium coatneyi reveals insights into barriers to human infection.

International journal for parasitology·2026
Same author

NVX-CoV2372, monovalent mRNA and bivalent mRNA vaccines elicit broadly cross-reactive antibodies against emerging SARS-CoV-2 variants.

Human vaccines & immunotherapeutics·2026
Same author

Optimizing mouse models for mRNA vaccines: addressing dose translation challenges.

Scientific reports·2026
Same author

Spatial predictors of response to chemo-immunotherapy in microsatellite stable metastatic colorectal cancer.

Nature communications·2026
Same author

Vaccine-induced mouse antibodies targeting Plasmodium falciparum PfVFT antigen inhibit blood stages through multiple mechanisms.

NPJ vaccines·2026

Related Experiment Video

Updated: Nov 15, 2025

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

9.1K

Microscopy-based Methods for Rosetting Assay in Malaria Research.

Wenn-Chyau Lee1, Laurent Rénia1

  • 1Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR), Singapore.

Bio-Protocol
|March 4, 2021
PubMed
Summary

Rosetting in malaria, where infected red blood cells bind to uninfected ones, is key to parasite survival. Standardized microscopy methods ensure consistent detection of this crucial malaria phenomenon.

Keywords:
Live intracellular parasite stainingMalariaMicroscopeRosettesWet mount

More Related Videos

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

8.2K
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
10:38

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

Published on: July 17, 2014

23.4K

Related Experiment Videos

Last Updated: Nov 15, 2025

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

9.1K
Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

8.2K
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
10:38

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

Published on: July 17, 2014

23.4K

Area of Science:

  • Parasitology
  • Immunology
  • Pathology

Background:

  • Rosetting, the adherence of Plasmodium-infected erythrocytes to uninfected ones, is observed in all human malaria species.
  • This phenomenon is a potential immune evasion strategy for Plasmodium parasites.
  • Rosetting contributes to malaria pathogenesis by potentially causing microvasculature occlusion and direct endothelial cytoadherence.

Purpose of the Study:

  • To evaluate microscopy-based techniques for visualizing malaria rosettes.
  • To establish standardized methods for consistent rosetting assays.
  • To present three reliable techniques for rosetting experiments.

Main Methods:

  • Comparison of microscopy-based techniques for rosetting visualization.
  • Development of preparation steps for consistent experimental performance.
  • Presentation of unstained wet mount, acridine orange fluorescence microscopy, and Giemsa stained wet mount methods.

Main Results:

  • No single microscopy technique is currently the "gold standard" for rosetting assays.
  • Microscopy techniques for rosetting can be used interchangeably under controlled experimental conditions.
  • Standardized preparation steps facilitate consistent performance in rosetting experiments.

Conclusions:

  • Standardized microscopy techniques provide reliable methods for malaria rosetting assays.
  • Consistent rosetting detection is achievable with proper experimental control.
  • The presented methods offer viable options for studying the rosetting phenomenon in malaria.