RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
1Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, United States of America.
Abstract:
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive method of mRNA stability measurement through quantitative reverse transcription PCR (RT-qPCR) after inhibition of RNA transcription by cordycepin in Arabidopsis seedlings.
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