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Intestinal Enteroid Culture for Human Astroviruses
Irene A Owusu1,2, Carmen Mirabelli1, Abimbola O Kolawole3
1Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
Bio-Protocol
|March 4, 2021
Summary
Human astroviruses (HAstV) cause gastroenteritis and neurological diseases. Human intestinal enteroids (HIE) provide a physiologically relevant model for studying all HAstV clades, aiding therapeutic development.
Area of Science:
- Virology
- Gastroenterology
- Human Intestinal Biology
Background:
- Human astroviruses (HAstV) are RNA viruses causing gastroenteritis and potential neurological diseases.
- Studying HAstV is crucial for understanding pathogenesis and developing treatments.
Purpose of the Study:
- To describe HAstV infection in epithelium-only human intestinal enteroids (HIE).
- To establish HIE as a physiologically relevant model for HAstV research.
Main Methods:
- Utilized epithelium-only human intestinal enteroids (HIE) derived from intestinal crypts.
- Inoculated HIE with various human astrovirus clades, including clinical samples.
Main Results:
- Human intestinal enteroids (HIE) support the replication of all tested HAstV clades.
- HIE serve as a physiologically relevant model, mimicking the human intestine.
Conclusions:
- Human intestinal enteroids (HIE) represent a valuable universal model for studying human astrovirus biology.
- This model facilitates research into HAstV pathogenesis and the development of therapeutics.
